CNS5:Diffuse hemispheric glioma, H3 G34-mutant: Difference between revisions
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==Genetic Diagnostic Testing Methods== | ==Genetic Diagnostic Testing Methods== | ||
<br /> | * Targeted sequencing to identify c.103G>A p.G35R (G34R), c.103G>C p.G35R (G34R), or c.104G>T p.G35V (G34V) is diagnostic for G34-DHG. | ||
* Pan-cancer sequencing will likely detect concurrent mutations in TP53, ATRX, PDGFRA etc. | |||
* DNA methylation and gene expression profiling can be used to differentiate G34-DHG with other glioma subgroups. | |||
* MGMT promoter methylation can be assessed by methylation specific polymerase chain reaction analysis (Bisulfite treated DNA undergoes real-time PCR)<br /> | |||
==Familial Forms== | ==Familial Forms== | ||