HAEM5:Systemic chronic active EBV disease: Difference between revisions
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** Whole blood or peripheral blood mononuclear cells are preferred for EBV DNA PCR testing, as serum or plasma are less sensitive for CAEBV disease<ref>{{Cite journal|last=Kimura|first=Hiroshi|last2=Cohen|first2=Jeffrey I.|date=2017|title=Chronic Active Epstein-Barr Virus Disease|url=https://pubmed.ncbi.nlm.nih.gov/29375552|journal=Frontiers in Immunology|volume=8|pages=1867|doi=10.3389/fimmu.2017.01867|issn=1664-3224|pmc=5770746|pmid=29375552}}</ref> | ** Whole blood or peripheral blood mononuclear cells are preferred for EBV DNA PCR testing, as serum or plasma are less sensitive for CAEBV disease<ref>{{Cite journal|last=Kimura|first=Hiroshi|last2=Cohen|first2=Jeffrey I.|date=2017|title=Chronic Active Epstein-Barr Virus Disease|url=https://pubmed.ncbi.nlm.nih.gov/29375552|journal=Frontiers in Immunology|volume=8|pages=1867|doi=10.3389/fimmu.2017.01867|issn=1664-3224|pmc=5770746|pmid=29375552}}</ref> | ||
** In tissues, using a double stain for B, T, or NK-cell markers and EBV is recommended. | ** In tissues, using a double stain for B, T, or NK-cell markers and EBV is recommended. | ||
* TCR-gene rearrangements can be tested via PCR | * TCR-gene rearrangements can be tested via PCR or NGS methods. | ||
* DNA mutations (SNV/Indels) can be detected using NGS | * DNA mutations (SNV/Indels) can be detected using NGS | ||